【广东会GDH基因检测】YAP/TAZ在角囊性牙源性肿瘤中的表达及其与增殖行为的可能关联

人体基因检测需要多少钱—比较

开会学习基因实验室人员知识更新《肿瘤靶向药物选择的基因突变标准》《Biomed Res Int》在. 2017;2017:4624890.发表了一篇题目为《YAP/TAZ在角囊性牙源性肿瘤中的表达及其与增殖行为的可能关联》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Qi-Wen Man , Yan-Qi Ma , Jin-Yuan Liu , Yi Zhao , Bing Liu , Yi-Fang Zhao 等完成。促进了肿瘤的正确治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤遗传的可能性分析临床研究内容关键词：

角囊性牙源性肿瘤,KCOT,口腔科肿瘤,YAP,TAZ

肿瘤靶向治疗基因检测临床应用结果

口腔科肿瘤基因检测研究的目的是阐明 YAP/TAZ 是否参与角囊性牙源性肿瘤 (KCOT) 的发病机制和增殖性生长，以便优化口腔癌肿瘤基因检测的基因及基因位点的选择及结果解读。通过免疫组化和实时定量PCR检测和比较正常口腔黏膜（OM）和角囊性牙源性肿瘤 (KCOT)中YAP/TAZ及下游蛋白和基因的表达水平。肿瘤基因检测结果表明，与口腔黏膜（OM）相比，角囊性牙源性肿瘤 (KCOT) 中 YAP/TAZ 和下游蛋白（Cyr61、CTGF）的表达显着上调，Ki-67 上调。重要的是，与转录共激活因子 YAP/TAZ 相互作用的转录因子（TEAD1、TEAD4 和 RUNX2）和细胞周期相关基因（CDK2、PCNA）的 mRNA 水平也在 KCOT 中上调。此外，Spearman 秩相关检验的结果揭示了 YAP/TAZ 和 Ki-67 之间的密切关系，双标记免疫荧光进一步证明了这一点，揭示了 KCOT 样品中 YAP/TAZ 与 Ki-67 的同步分布。所有数据表明 YAP/TAZ 可能与角囊性牙源性肿瘤 (KCOT) 的增殖行为有关。

肿瘤发生与反复转移国际数据库描述：

The aim of this study is to clarify whether YAP/TAZ is involved in the pathogenesis and proliferative growth of keratocystic odontogenic tumor (KCOT). The expression levels of YAP/TAZ and downstream proteins and genes in normal oral mucosa (OM) and KCOT were determined and compared by immunohistochemistry and real-time quantitative PCR. The results showed that the expression of YAP/TAZ and downstream proteins (Cyr61, CTGF) was significantly upregulated in KCOT with upregulation of Ki-67 compared to OM. Importantly, the mRNA levels of transcription factors (TEAD1, TEAD4, and RUNX2) and cell cycle related genes (CDK2, PCNA), which interact with the transcriptional coactivators YAP/TAZ, are also upregulated in the KCOT. In addition, the results from Spearman rank correlation test revealed the close relationship between YAP/TAZ and Ki-67, which was further evidenced by double-labelling immunofluorescence that revealed a synchronous distribution for YAP/TAZ with Ki-67 in KCOT samples. All the data suggested YAP/TAZ might be involved in the proliferative behavior of KCOT.